HPLC法测定二氢杨梅素含量的操作规程

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HPLC法测定二氢杨梅素含量的操作规程

起草单位：长沙上禾生物科技有限公司

目的：HPLC法测定二氢杨梅素含量的操作规程

责任人：QC员。

内容：

1、试剂

1.1 甲醇（HPLC级）

1.2 蒸馏水（AR级）

1.3冰醋酸（HPLC级）

2、对照品

二氢杨梅素（购自中国药品生物制品检定所）

3、仪器和用具

3.1 电子天平（1/100000）

3.2 玻璃仪器：100ml容量瓶

3.3 安捷伦1220 HPLC

4、液相条件

4.1流动相：甲醇：水：冰醋酸=50:50:1

4.2检测波长：292nm

4.3流速：1ml/min

4.4柱温：30℃

5 、操作步骤

5.1 对照品溶液的制备精密称取在110℃干燥至恒重的二氢杨梅素对照品10mg，置50ml容量瓶中，加50%甲醇适量，超声处理10min使溶解，放冷，加50%甲醇至刻度，摇匀，备用。

5.2样品溶液制备精密称取在110℃干燥至恒重的二氢杨梅素样品10mg，置50ml容量瓶中，加50%甲醇适量，超声处理10min使溶解，放冷，加50%甲醇至刻度，摇匀，备用。

6、检测

取对照品溶液和样品溶液0.22μm针孔过滤器过滤，进样20μl，根据峰面积计算样品含量。

含量按以下公式计算：

A2 x C1 x T

R=------------------------- x 100

A1 x C2

C1: 对照品的参考浓度，mg/ml

C2: 样品的参考浓度，mg/ml

A1: 对照品溶液的色谱主峰值面积

A2: 样品溶液的色谱主峰值面积

T: 对照品的含量

藤茶生产工艺流程
藤茶提取物-二氢杨梅素生产工艺流程：

原料→挑洗→去杂→粉碎→乙醇提取→浓缩→离心→上大孔树脂柱→20%,40%,60%,80%乙醇分别洗脱
→分段收集→脱色→结晶→干燥→混合、筛分、包装→检测合格后入库

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